A systematic study of field inversion gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Epidemiologic study of Taylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments.
Contagious equine metritis (CEM), a sexually transmitted bacterial disease, was first described in thoroughbred horses. It also occurs in nonthoroughbred horses, in which it produces isolated, apparently unrelated outbreaks. Thirty-two strains of Taylorella equigenitalis, the causative agent of CEM, from all over the world were characterized by field inversion gel electrophoresis of fragments o...
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Field inversion gel electrophoresis (FIGE) was performed on 139 isolates of group A streptococcus (GAS) representing 72 different M types. Multiple isolates were tested for 27 M types (range, 2-19). Each of the M types tested had at least one unique banding pattern that was not observed in any other M type. For 23 of 27 M types for which multiple isolates were available, band profiles of the is...
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Pulsed field gel electrophoresis (PFGE) is capable of resolving large DNA molecules of up to two million nucleotides and is becoming a widely used technique in DNA studies (1—3). Three factors can potentially limit PFGE in a laboratory: 1. the cost of the apparatus, 2. reproducibility of electrophoretic conditions, and 3. unusually long electrophoretic time. To alleviate some of these problems ...
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By making use of preparative field inversion gel electrophoresis, we have constructed a lambda ZAP library that is highly enriched for sequences from the choroideremia locus. In vivo excision of pBluescript SK(-) constructs from lambda ZAP obviates the subcloning of DNA inserts and allows for rapid processing of several hundred recombinants. From a 625 kb Sfil fragment we isolated 7 clones that...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.15.5989